Acta Universitatis Sapientiae, Agriculture and Environment, 17 (2025) 38–49
Abstract: Biochar is a charcoal-like organic product formed by pyrolysis, which acts as a soil amendment that boosts fertility and supports PGPR colonization. This study involves the production of garden-waste-derived biochar that enhances soil fertility and supports PGPR in tomato rhizospheres. Preliminary biochemical assays were performed to identify the strains and were authenticated with 16S rRNA sequencing studies. Two key strains, Burkholderia cepacia and Bacillus tropicus, were found in both treated and untreated soils. The physiological growth parameters of plant trials validate the fertility of enriched biochar and establish it as a potential bio-fertilizer source to improve crop health.
Keywords: bio-fertilizer, garden waste, pyrolysis, soil fertility, Burkholderia cepacian, Bacillus tropicus

SAPIENTIA HUNGARIAN UNIVERSITY OF TRANSYLVANIA
The Sapientia Hungarian University of Transylvania is the independent university of the Hungarian community in Romania, which aims at providing education to the members of our community and performing scientific research on a high professional level.
Sapientia Hungarian University of Transylvania,
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Acta Universitatis Sapientiae
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Email: acta @ acta.sapientia.ro